Muraoka, and M. Bacterial communities associated with nodal roots of rice plants along with the growth stages: estimation by PCR—DGGE and sequence analyses. Soil Sci Plant Nutr. Marilley, L. Phylogenetic diversity of bacterial communities differing in degree of proximity of Lolium perenne and Trifolium repens roots. Appl Soil Ecol. Yang, C. Rhizosphere microbial community structure in relation to root location and plant iron nutritional status.
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Appl Environ Microbiol. Arenz, B. Schlatter, J. Bradeen, and L.
Blocking primers reduce co—amplification of plant DNA when studying bacterial endophyte communities. J Microbiol Meth. Lundberg, D. Yourstone, P. Mieczkowski, C. Jones, and J. Practical innovations for high—throughput amplicon sequencing. Nat Methods. Vestheim, H.
Front Zool. Ikeda, S.
Fuji, T. Sato, N.
Ytow, H. Ezura, and K. Community analysis of seed—associated microbes in forage crops using culture—independent methods. Microbes Environ. Sakai, M. Application of peptide nucleic acid PNA —PCR clamping technique to investigate the community structures of rhizobacteria associated with plant roots. Kawauchi, and M. Culture—independent molecular technique to investigate the community structures of plant—associated bacteria, and the potential to develop the related research.
Soil Microorganisms. In Japanese.
Adapt to plants | Nature Plants
Yu, Z. Yu, M. Sakai, X. Liu, and G. Characterization of root—associated bacterial community structures in soybean and corn using locked nucleic acid LNA oligonucleotide—PCR clamping and pyrosequencing. J Integr Agric. Tabuchi, T. Oyama, I. Akagi, and M.
Swarming motility in plant-associated bacteria
Development of LNA oligonucleotide—PCR clamping technique in investigating the community structures of plant—associated bacteria. Biosci, Biotechnol, Biochem. The sequences of type strains were mainly collected. Bacterial genes were aligned together with modified 63f or r.
The numbers of bacterial genes used for alignment were 12, and 14, for the forward and reverse sides, respectively. Based on alignment, three new forward primers, KU63f, KU64f, and KU68f, were designed by improving the modified 63f primer at the position to compete with LNA oligonucleotides on the forward side Fig. Properties of newly designed bacterial primers, which were designed by improving the original primer set of modified 63f and r.
Ashelford, K. Weightman, and J.
Nucleic Acids Res. Rice Oryza sativa cv. Koshihikari , potato Solanum tuberosum cv.
Nishiyutaka , soybean Glycine max cv. Fukuyutaka , and turnip green Brassica rapa var. Cultivation procedures were the same as those described by Ikenaga et al. As shown in Fig. Klindworth, A.
Pruesse, T. Schweer, J. Peplies, C. Quast, M. Horn, and F. Caporaso, J. Kuczynski, J. Stombaugh, et al. QIIME allows analysis of high—throughput community sequencing data. Bru, D. It seems that you're in Germany. We have a dedicated site for Germany. This volume is developed on the broad theme of plant-associated bacteria. It is envisioned as a resource volume for researchers working with beneficial and harmful groups of bacteria associated with crop plants.