Peptide Science, Present and Future

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Peptide conformational behaviour studied by high and low temperature reversed phase HPLC. Three-dimensional structure and receptor-recognition sites of bombyxin-II, an insulin-like brain-secretory peptide of the silkmoth. Conformational analysis of peptide fragments corresponding to domains of potassium ion-channel proteins.

Peptide Synthesis: An Essential Tool for Chemical Biology

Solution structure of the antimicrobial peptide ranalexin and a study of its interaction with dodecylphosphocholine micelles. Formation of native disulphide bonds in endothelin evidence for the involvement of a highly specific salt-bridge between the pro-sequence of endothelin-1 and mature sequence. Structure-biological activity relationships of cecropin A 1—8 -magainin 2 1—12 hybrid and its analogues. Structure analysis of the second transmembrane segment of the rat bradykinin receptor in solution and in micelles by CD and fluorescence spectroscopies.

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Conformational analysis of antimicrobial peptide tachystatin A isolated from horseshoe crab hemocytes by two dimensional 1H NMR spectroscopy. Synthesis of peptides containing 9-aminofluorene- 9-carboxylic acid by the modified Ugi reaction. Comparison of three-dimensional solution structures of dendrotoxin I, a potassium channel blocker, and calucicludine, a calcium channel blocker, determined by nuclear magnetic resonance spectroscopy.

Sequence, conformation, and function of peptides, retro peptides and sense and antisense gene products. Ion conductance in a gramicidin channel: the right-handed double-stranded double-helical dimer. Topological isomers of human uroguanylin: structure determination by nuclear magnetic resonance. Homology modelling of the glycoprotein hormones: examination of the receptor recognition characteristics. Prion protein structure and pathology of transmissible spongiform encephalopathies TSE. Peptides and molecular recognition: head-to-tail self-assembly, formation of amphipathic surfaces and recognition of anionic superhelices.

Characterization and evolutionary aspect of a Trimeresurus flavoviridis serum inhibitor, PLI-I, against its venom basic phospholipase A2 isozymes. A 24 mer peptide with activities of discrimination and recombination of nucleic acids. Phosphorylation of synthetic peptides containing proline homologs by cdc2 kinase or cdk5. Synthesis of oligomerization domain of p53 and its analogs and their association properties in buffer solution.

Stabilization of tyrosine-O-sulfate residues through the formation of a conjugate acid-base pair.


The role of nucleoplasmin — molecular chaperone protein — in the fertilization process. The two adenosine triphosphate and nucleic acid binding sites of the calf thymus single-strand DNA-dependent adenosine triphosphatase. Effect of carnosine and its natural derivatives on apoptosis of neurons induced by excitotoxic compounds. Morphological differences observed by the different modes of action of the antibacterial peptides, cecropin B and its analogs CB-1, CB-2 and CB-3 on bacterium and cancer cell.

Isolation and characterization of bioactive peptides from the sea cucumber, Stichopus japonicus. Purification of peptide fractions with anticomplementary activity from red ginseng Panax ginseng, C. Isolation and cDNA cloning of oryctin, a novel cysteine-rich antibacterial peptide, from the coconut rhinoceros beetle, Oryctes rhinoceros.

Eumenine mastoparans. A new class of mast cell degranulating peptides in the eumenine wasp venom. Human immunodeficiency virus type 1 Vif-derived peptides inhibit the viral protease and arrest virus production. Chemical and biological characterization of neuropeptides in the sinus glands of the kuruma prawn, Penaeus japonicus. Preparation of a poly ethylene glycol hybrid containing two active fragments of laminin. Modification of an anti-fungal peptide ESF-1 for enhanced inhibitory activity and resistance to proteolysis. Isolation and characterization of a bacteriocin-like peptide produced by Brevibacterium linens ATCC , an orange cheese coryneform bacterium.

Synthesis, conformational analysis, and biological activity of opioid peptide analogs containing side chain fluorinated amino acids. Structural requirement tryptophan1,3 of tridecapeptide mating pheromone of Saccharomyces cerevisiae.

Peptide Science - Present and Future

Macrotorials: synthesis and characterization of model compounds related to PTH 1— Developments in peptide synthesis and peptidomimetic chemistry: HIV protease analogs and substrate-based inhibitors. Synthesis of disulfide-bridged heterotrimeric collagen peptides.

Conformational properties and digestion by matrix metalloproteinases. A simple linker for the attachment of aldehydes to the solid phase. Synthesis of protected 2,3-epoxy amines utilizing aza-Payne rearrangement of activated 2-aziridinemethanols under basic conditions. Convenient preparation of phosphopeptide fragments using High TFMSA system and its application to synthesis of long chain phosphopeptides. An efficient synthesis of tryptophan-containing cystine peptide using the silyl chloride-sulfoxide system. Synthesis of an HIV-1 protease dimer analog covalently linked by a disulfide bridge.

Facile synthesis of the acid-labile peptide amide linker containing the 10, dihydro-5H-dibenzo [a, d] cycloheptenyl group. Synthetic approach to antillatoxin, an exceptionally ichthyotoxic cyclic lipodepsipeptide. Desulfation vs. Studies of racemization in peptide synthesis for a phosphoryloxy-benzotriazin-one coupling reagent DEPBT. Effect of polyethylene glycol on the synthesis of oligopeptides by proteases in an organic medium.

Highly efficient solid phase cyclization of peptides by methylenedithioether or disulfide bond formation. Total synthesis of human pleiotrophin, a residue heparin-binding neurotrophic factor having five disulfide bonds. A facile preparation of 2,2-difluoro-1,3,2-oxazaborolidin- 5-ones I and their application to side-chain protection of Asp, Glu, Ser and Thr.

ESR-resin solvation approach for the investigation of the highly peptide-loaded synthesis protocol. Synthesis of reaper protein by a thioester method using silver chloride as an activator. Preparation of a peptide thioester by a 9-fluorenylmethoxycarbonyl solid phase method. Chemical synthesis of barnase which possesses phosphoserine residue in place of the catalytic glutamic acid at position Chemo-enzymatic syntheses of eel calcitonin analogs having natural N-linked oligosaccharides.

Chemo-enzymatic synthesis of bioactive peptide derivatives containing N-linked oligosaccharides.

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Solid phase synthesis of O-glycopeptide — Preparation of O - N-acetylgalactosaminyl -threonine derivative and its application to peptide synthesis. Design and synthesis of potent and selective inhibitors of the osteoclast-specific cysteine protease, cathepsin K. Difference of structural requisite in design of inhibitor of cathepsin B and papain: X-ray crystal structures of complexes. Isolation and characterization of prolyl endopeptidase-inhibiting peptides from bovine brain.

Suicide-inhibition of human immunodeficiency virus type-1 protease by the gag precursor processing product p2gag peptide. Design of plasma kallikrein selective inhibitor and its application to affinity chromatography. Allophenylnorstatine containing HIV-1 protease inhibitors: design, synthesis and structure-activity relationships for selected P2 ligands.

Frontiers of Peptide Science

Opioid peptide analogs with novel activity profiles as potential therapeutic agents for use in analgesia. Peptide radiopharmaceuticals: studies of somatostatin receptors and ligands for tumor targeting. Fatty acid acylated insulins display protracted action due to binding to serum albumin. Melanotropin fragments containing difluoromethylornithine: synthesis and cytotoxicity. The influence of structural motifs of amphipathic peptides on the permeabilization of lipid bilayers and the antibacterial and hemolytic activity.

Proline at position 14 of alamethicin is essential for the hemolytic activity, catecholamine secretion from chromaffin cells and enhanced metabolic activity in endothelial cells. Synthetic antibacterial peptides derived from insect defensin isolated from a beetle, Allomyrina dichotoma. Incorporation of the nuclear localization signal NLS peptide-albumin conjugate to macrophages using pH-sensitive liposomes.

Dermorphin-dynorphin hybrid analogs: Opioid receptor selection and enzymatic stability. Synthesis and biological activities of hPTH 1—34 analogues: modification of the middle part and C-terminal alkylamides. Absolute importance of arginine residue at position 7 of dog neuromedin U-8 for contractile activity.

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Importance of hydrophobic region in cationic peptides on gene transfer into cells and enhance effect of lysosome-disruptive peptide. Synthesis and pharmacological properties of oxytocin antagonists containing conformationally constrained amino acids. Identification of the structural elements responsible for high biological activity of dimeric opioid peptide biphalin.

Orally active memory-enhancing peptides obtained by designing bioactive peptides derived from food proteins. The structure—activity relationships of the sweet protein brazzein — roles of acidic amino acids. Comparison of secondary structure and antibacterial activity of bactericidal peptides derived from bovine and Korean native goat lactoferrin. Improvements of activity and stability against proteases of biologically active peptides by coupling of a cyclic peptide derived from an endothelin antagonist RES Coacervation of elastomeric polypeptides: microscopic observations and light scattering measurements.

Preparation and properties of novel gramicidin S analogs possessing a polymethylene bridge between ornithine side chains. Effect of nitroprusside on amyloid peptide induced inhibition of MTT reduction in pheochromocytoma PC12h and C neuroblastoma cells.

Practical approach to enzymatic resolution of Fmoc- and Boc-protected amino acid racemates. Combinatorial peptide libraries and molecular recognition in T-cell mediated immune response. Design of peptidic and non-peptidic CD4 inhibitors as novel immunotherapeutic agents [1—5]. Contribution of peptide backbone atoms to binding of an antigenic peptide to class I major histocompatibility complex molecule.

B cell and T cell epitopes of Der f 2, a house dust mite major allergen of the Dermatophagoides farinae. Conformational and binding studies of the peptide isolated from phage display library by anti-mouse CTLA-4 monoclonal antibody.

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Evaluation of region-specific antibodies for rat and human Chromogranin A with use of synthetic peptide. Design of synthetic antigen containing non-immunogenic T cell epitope core and simple non-native flanking regions. Immune response to newly exposed regions of Band 3 protein of the red blood cell membrane during malaria infections.